Basic Microscopy 'In the Footsteps of Antoni van Leeuwenhoek'

This course teaches you the application of a range of imaging possibilities within the Centre for Microscopy at the Amsterdam UMC (location AMC & VUmc) and the NKI-AvL. They are presented in lectures, discussions and hands-on demonstrations. The individual research projects of the attending participants will be discussed in relation to the demonstrated techniques, allowing exchange of ideas with fellow participants and microscopy experts and operators. The course is a guide to implement cellular imaging in your own research project. A part of the participants could bring their sample for analysis after consulting with the organization first.

Topics covered include
Basic principles of microscopy
Specimen preparation, staining methods and assay systems
Quantitative analysis of microscopic images  
Confocal laser scanning microscopy
Electron microscopy techniques
Imaging flow cytometry
Wide field microscopy
Ultramicroscoop and 2-photon
Live cell imaging 

Target audience:


Registration deadline:

November 27 – December 1, 2023
AMC, NKI-AvL and VUmc
OOA PhD students. All other interested PhD students, postdocs and technicians with their own research project can also attend when places are available.
Free of charge
J Belien, L Brocks, A Dick,  M Mertz, E Reits, N van der Wel
Esther Ruhé (
October 16 , 2023