Basics in CRISPR
Welcome to the 2022 OOA Basics in CRISPR Course!
For this edition, we designed a program for researchers that have little know-how and experience with CRISPR but would like to learn how to use this technology in their research.
CRISPR genome editing is revolutionizing biomedical research, providing a toolbox for precise modification of genomic sequences and their regulation in any type of cell and organism. The application of CRISPR in biomedical research is broad and ranges from fundamental research to the clinic.
During this course participants will be introduced to basic concepts of CRISPR-mediated genome editing, and hands-on experimental design and protocols. In a series of short presentations and open discussion sessions, the program is focused on breaking down the steps needed to start using the most common CRISPR applications.
After finishing this course you will have acquired broad knowledge on the basics of CRISPR genome editing, the design of CRISPR experiments and diverse applications of CRISPR genome editing. You will be able to apply this knowledge to advance your own research projects.
The preliminary program:
Day 1: 09.00-17.00 Introduction, Design your guide, Make a knock-out
Day 2: 10.30-16.00 Make a knock-in, Edit a base
Day 3: 09.00-17.00 CRISPR screening
Day 4: 13.30-17.00 Mouse models, in vivo base-editing, CRISPR therapeutics
On each day there will be assignments to gain experience with the different tools available for CRISPR based technologies
The course is organized and coordinated by The Netherlands Cancer Institute, Amsterdam UMC Cancer Center Amsterdam and the ScreeninC national infrastructure for genome editing and large scale functional genomic screening, in collaboration with the OOA.
Date:
Location:
Target audience:
Price:
ECTS:
Organization:
Information:
Registration:
Deadline:
5 – 8 April, 2022
NKI-AvL
OOA PhD candidates CCA Amsterdam UMC and NKI-AvL
Free of charge
1.0
Roderick Beijersbergen, Rodrigo Gomes Leite de Oliveira
Patty Lagerweij: p.lagerweij@nki.nl
The course is limited to 28 participants. Please register promptly!
March 4, 2022